Rapid Plasma Reagin test was used in four studies,14, 15, 32, 33 six articles14, 17, 18, 24, 33, 40 used IgG, IgM, and IgA antibodies against Treponema pallidum, Treponema pallidum Micro‐hemagglutination assay14, 15 was used in two research studies and finally, Venereal Disease Research Laboratory17, 40 was used in two articles to detect syphilis infection. The gene discussed is CD40LG; the disease is glycogen storage disease VI.