As shown in Figure 4, the qRT−PCR results indicated that four of six candidate mRNAs, namely, ENSG00000198763 (MT-ND2) (P=2.9e-10), ENSG00000198786 (MT-ND5) (P=1.8e-07), ENSG00000198840 (MT-ND3) (P=1.2e-08) and ENSG00000269028 (MTRNR2L12) (P=5.8e-10), were significantly upregulated in T1DM patients compared with control subjects, demonstrating the predictive accuracy of the sequencing data and the biomarker potential of exosomal mRNAs. This evidence concerns the gene MT-ND2 and type 1 diabetes mellitus.