To further assess the biomarker potential for T1DM, six exosomal DEMs, including five upregulated DEMs, ENSG00000185883 (ATP6VOC), ENSG00000198763 (MT-ND2), ENSG00000198786 (MT-ND5), ENSG00000198840 (MT-ND3) and ENSG00000269028 (MTRNR2L12), and one downregulated DEM, ENSG00000158417 (MTRNR2L12), were selected to validate the sequencing data in an independent cohort. This evidence concerns the gene MT-ND5 and type 1 diabetes mellitus.