As shown in Figure 4, the qRT−PCR results indicated that four of six candidate mRNAs, namely, ENSG00000198763 (MT-ND2) (P=2.9e-10), ENSG00000198786 (MT-ND5) (P=1.8e-07), ENSG00000198840 (MT-ND3) (P=1.2e-08) and ENSG00000269028 (MTRNR2L12) (P=5.8e-10), were significantly upregulated in T1DM patients compared with control subjects, demonstrating the predictive accuracy of the sequencing data and the biomarker potential of exosomal mRNAs. The gene discussed is MT-ND5; the disease is type 1 diabetes mellitus.