To further pinpoint specific methylation sites, we used the MethSurv-A database to find the three sites with the highest methylation levels in PLK4, including cg22112850, cg06015521, and cg26882168, among which, the cg22112850 site had the highest methylation level, and analysis of its relationship with cancer prognosis revealed that the higher the methylation level of cg22112850 site, the better it was for cancer prognosis, and the cg22112850 site methylation might be beneficial for cancer prognosis. Here, PLK4 is linked to cancer.