Our results revealed more CD3 and CD4 T cell infiltration in the skin from the lymphedema hind limbs than in the skin from the sham control group (CD3: 24.13 ± 4.29 cells/high‐power field [HPF] vs. 8.30 ± 5.63 cells/HPF; CD4: 52.33 ± 28.44 cells/HPF vs. 19.13 ± 11.66 cells/HPF, p < 0.0001) and that there was no significant difference between the lymphedema hindlimb receiving VLNT and the skin from the sham control group (CD3: 12.00 ± 5.94 cells/HPF vs. 8.30 ± 5.63 cells/HPF; CD4: 30.33 ± 13.33 cells/HPF vs. 19.13 ± 11.66 cells/HPF, p < 0.0001). The gene discussed is CD4; the disease is lymphedema.