However, considering that the diagnosis of diseases should not rely on a single indicator, but often requires the joint diagnosis of multiple indicators, we incorporated nine hub genes into the NSCLC diagnosis system, fitted a regression equation (z = 0.031* BUB1B+ 2.666* CDC20 + 0.138* KIF11 + 0.879* BUB1+ 2.224* CDC6- 1.922* CDK1- 0.793* CCNA2+ 2.052* KIAA0101–0.983* NCAPG- 26.192) for the diagnosis score of NSCLC through logistic regression analysis, and conducted ROC analysis on the prediction probability of the regression analysis (Figure 5G). The gene discussed is CDC20; the disease is non-small cell lung carcinoma.