ROC curve of independent factors showed that all nine hub genes had moderate or above diagnostic value for NSCLC (Figures 5B–F), among which CDC20, BUB1B, BUB1 and KIF11 had significant diagnostic value, and the areas under the curve were 0.9594 [95% CI: 0.9413 to 0.9775], 0.95 [95% CI: 0.9284 to 0.9716], 0.9318 [95% CI: 0.9069 to 0.9567] and 0.9111 [95% CI: 0.8816 to 0.9406], respectively (p < 0.0001). This evidence concerns the gene BUB1B and non-small cell lung carcinoma.