Cytochrome C protein expression levels were found to be lower in Glioma tissues than in normal tissues, and the number of positive cells declined as the grade of Glioma rose (Grade I 53% ± 2.10, Grade II 44% ± 2.45, Grade III 31% ± 6.10, and Grade IV 18% ± 4.16). This evidence concerns the gene CYCS and central nervous system cancer.