We supplemented this analysis with differential splicing analysis of RNAseq data from 3 independent mutant PRPF31+/− hTERT-RPE1 clones and 3 independent wild-type sister hTERT-RPE1 clones, which we previously described (Nazlamova et al., 2020), and publicly available RNAseq data from day 150 retinal organoids derived from induced pluripotent stem cells (iPSCs) from 7 PRPF31 patients severely affected with RP, compared to 6 unaffected controls (Buskin et al., 2018). Here, PRPF31 is linked to retinitis pigmentosa 1.