The RT-qPCR results indicated that the mRNA expression of genes associated with barrier integrity, including ZO-1, the CLDN family, and the MUC family, decreased significantly in DSS-induced colitis, but were rescued in the colons of miR-200b-3p- or miR-181b-5p-treated mice (Figure 3o), especially the expression of CLDN1, CLDN2, MUC1 and MUC4. This evidence concerns the gene CLDN1 and colitis.