As shown in Additional file 1: Fig. S1D, the expressions of GSDMC, GSDMD and GSDME were markedly higher in H1688 and A549 cells compared with those in BEAS-2B cells and other 9 LC cell lines, supporting our in vitro results that H1688 and A549 cells were more sensitive to NC treatment. The gene discussed is GSDME; the disease is laryngotracheoesophageal cleft.