Vaccination with Tm-WAP49 formulated with Montanide ISA 720 showed efficacy (48% reduction in worm burden) against a high-dose infection in AKR mice, and this was attributed to the induction of antigen-specific Th2 immunity (high levels of IL-5 and IL-13 secretion by splenocytes, and lymphocytes in the vaccine-draining [inguinal] and parasite-draining [mesenteric] lymph nodes) [45]. This evidence concerns the gene IL13 and infection.