Assuming the following candidate genes [CTSZ, EFEMP2, ITGA5, KDELR2, MAP2K3, MDK, MICALL2, PLAUR, SERPINE1, SOCS3], a tumor cohort including all skin cutaneous melanoma patients with somatic mutations aligned to GRCh38, and a healthy cohort including all donors having germline variants aligned to GRCh38, using VarSum we can easily compute the gene scores and their rank normalization, shown in Fig. 7. The gene discussed is ITGA5; the disease is cutaneous melanoma.