Extensive adipocyte differentiation was confirmed by Oil Red O staining and expression of adipocyte markers FABP4 and LEP, and there were no differences when comparing differentiated visceral adipose tissue-derived cells (Vis AD) and differentiated subcutaneous adipose tissue-derived cells (Sub AD) (Supplementary Fig. 2a–c), thereby excluding a potential differentiation bias. This evidence concerns the gene LEP and Alzheimer disease.