We purified TME myeloid cells from primary GBM cultures (n=7 independent samples) using CD11b magnetic activated cell sorting (MACS) (Figure 5A), which yielded live CD11b-positive GBM fractions (GBM11b+) enriched for a variety of IBA1+ myeloid cells (Figure S4F), and depleted for SOX2+ glioma cells, as well as CD11b-depleted GBM fractions (GBM11b-) exhibiting a higher SOX2/IBA1 ratio (Figure 5B; Figure S5A) compared to parental GBM cultures. The gene discussed is SOX2; the disease is glioblastoma.