There was obvious evidence of apoptosis with a significant increase in Bax (1.49-fold of Wt-Ctrl in wild-type mice, 1.50-fold of 3×Tg-Ctrl in 3×Tg-AD mice) and decrease in Bcl2 (0.86-fold of Wt-Ctrl in wild-type mice, 0.89-fold of 3×Tg-Ctrl in 3×Tg-AD mice) in the hippocampi, and predominant localization of TUNEL+ and cleaved caspase 3+ cells in the hippocampal CA3 region in both strains following laparotomy (Figure 3D, Supplementary Figure S2). This evidence concerns the gene CASP3 and Alzheimer disease.