Female age at blastocyst vitrified (p < 0.0001), basal AMH level (p < 0.0001), basal FSH level (p = 0.0085), the number of previous embryo transfer cycles (p < 0.0001), and proportion of male factor infertility (p = 0.0180) were significantly different between the day 5 blastocyst group and the day 6 blastocyst group. This evidence concerns the gene BRD2 and Infertility.