CD4 and cancer: Next, gene set enrichment analysis (GSEA) of the DGEs was performed according to KRAS/TP53 mutation status, and significantly downregulated metabolic regulating pathways, including Glycolysis, Cholesterol homeostasis, and Myc targets, were observed in CD4+ and CD8+ T cells from KRAS/TP53 dual mutant cancers (Supplementary Figures 4A–E).