Results of knockdown and knockout studies contain general descriptions of microphthalmia, coloboma, and anterior defects, but an in-depth understanding of mechanism or contribution of CHD7 to these defects is lacking (Jacobs-Mcdaniels and Albertson, 2011; Balow et al., 2013; Cloney et al., 2018; Liu et al., 2018; Liu and Liu, 2019; Breuer et al., 2020). Here, CHD7 is linked to coloboma.