As for the cellular signaling pathway, recombinant human REG4 (rhREG4) treatment resulted in anti-apoptosis of colorectal cancer cells with the overexpression of B cell lymphoma-extra large (Bcl-xL), B cell lymphoma 2 (Bcl-2), survivin, and matrix metalloproteinase-7 (MMP-7), and the phosphorylation of epidermal growth factor receptor (EGFR) at Tyr992 and Tyr1068, and Akt at Thr308 and Ser473. This evidence concerns the gene BCL2 and colorectal cancer.