Besides the markers related to liver damage, we also examined certain inflammatory factors including IL-17A, IL-1α and IL-1β in murine NAFLD model and results showed treatment with Rhein obviously suppressed the increase of IL-17A, IL-1α and IL-1β due to the high-fat diet in murine NAFLD model (Figures 5A–C). This evidence concerns the gene IL17A and metabolic dysfunction-associated steatotic liver disease.