In conclusion, we performed a comprehensive pan-cancer analysis of CREB3L1 by assessing multifaced aspects of CREB3L1, including the expression pattern, prognostic significance, genetic mutations, and immune-related signature, revealing the promising role of CREB3L1 as a promising indicator for the prognosis and immunotherapeutic efficacy of cancer patients and its immunoregulatory effects in pan-cancer. This evidence concerns the gene CREB3L1 and cancer.