In addition, western blot and qRT-PCR analysis showed that deficiency of Airn significantly promoted the upregulation of CCl4-induced α-SMA, COL1α1, MMP2, and TIMP1 (Fig. 2B, C), suggesting Airn deficiency aggravated CCl4-induced liver fibrosis. The gene discussed is MMP2; the disease is Hepatic fibrosis.