As shown in Additional file 1: Fig. S5A-C and Additional file 1: Table S3, the mice of BDL group developed serious liver fibrosis, and knockout of Airn further aggravated BDL-induced liver fibrosis as demonstrated by macroscopic examination, H&E staining, sirius red staining, IHC, serum ALT, AST level, liver hydroxyproline content, western blot, and qRT-PCR. This evidence concerns the gene AIRN and Hepatic fibrosis.