scRNA-seq of CD24–CD45– stromal cells from tumours in DTR– and DTR+ mice was carried out at 4 different time points, including 10 days after tumour implantation and immediately before initiation of DT treatment (IOT) (day 0) and on days 7, 14 and 21 after IOT (Fig. 3a and Extended Data Fig. 5a, top). This evidence concerns the gene PTPRC and neoplasm.