scRNA-seq was performed on CD24–CD45– stromal cells and CD45+ immune cells from both Dptwt/wtTgfbr2fl/fl and Dptki/kiTgfbr2fl/fl tumours (Extended Data Fig. 1c).After quality control, dimensionality reduction and clustering, four main groups of cells were identified, each represented by cells from multiple animals (Extended Data Fig. 1d). The gene discussed is CD24; the disease is neoplasm.