Immunohistochemical analysis for the expression of PD-1 and granzyme B by tumours obtained from mice across the different treatments showed that muPD1-IL2v induced a significantly higher number of PD-1+ (Fig. 3b) and granzyme B+ (Fig. 3c) tumour-infiltrating lymphocytes (TILs) than the other treatments. This evidence concerns the gene PDCD1 and neoplasm.