SAP130 and hydronephrosis: MRI, gross examination, and dissection of a CRISPR gene-edited SAP130 mutant term pig showed multiple malformations that consisted of an imperforated anus with rectal atresia (Fig. 7A–D), kidney defects when compared to normal age matched controls (Fig. 7E,F) including hypoplastic kidneys with one kidney showing very severe hydronephrosis with a thin rim of renal parenchyma and hydroureter and the other hypoplastic kidney showing a moderate hydroureter (Fig. 7G–J).