We identified four genes that met all three criteria: PRSS16 and MRM2, with increased expression in depression, and ZKSCAN8P1 and PCDH8, with decreased expression in depression, regulated by five MDD risk alleles involving rs72839477, rs67981811, rs12525684, rs2806933, and rs11772627 76. Here, PRSS16 is linked to depressive disorder.