We identified four genes that met all three criteria: PRSS16 and MRM2, with increased expression in depression, and ZKSCAN8P1 and PCDH8, with decreased expression in depression, regulated by five MDD risk alleles involving rs72839477, rs67981811, rs12525684, rs2806933, and rs11772627 76. This evidence concerns the gene ZKSCAN8P1 and major depressive disorder.