The AML-related genes including NRAS (1p13.2), MCL1 (1q21.2), EVI1 (3q26.2), GATA2 (3q21.3), WT1 (11p13), and PAK1 (11q14.1) were amplified in the eccDNAs of AML evolution compared with normal hematopoietic cells. The gene discussed is NRAS; the disease is acute myeloid leukemia.