The AML-related genes including NRAS (1p13.2), MCL1 (1q21.2), EVI1 (3q26.2), GATA2 (3q21.3), WT1 (11p13), and PAK1 (11q14.1) were amplified in the eccDNAs of AML evolution compared with normal hematopoietic cells. Here, MCL1 is linked to acute myeloid leukemia.