To explore the roles of NLGN1-AS1 and FZD4 in tumor growth in vivo, NLGN1-AS1 knockout resulted in a lower incidence of tumor and a significant reduction in tumor size compared with sh-NC+pcDNA3.1, whereas the results showed that FZD4 over-expression had a reversal of the effect of NLGN1-AS1 on tumor growth. Here, FZD4 is linked to neoplasm.