It has been reported that resorption of the bone matrix by activated osteoclasts could release various bone matrix‐bound factors, such as transforming growth factor beta (TGF‐β), which facilitates the seeding and expansion of metastatic tumor cells in bone, thus forming a “vicious cycle”.[15] We then further examined the TGF‐β level released from LOs‐treated bone slice and the effect on the proliferation of HCC cells. Here, TGFB1 is linked to neoplasm.