Importantly, cells overexpressing MIST1 were treated with Oroxin B, and these cells abrogated the effect of MIST1-mediated PTEN downregulation and p-AKT upregulation (Figure S5A), indicating that MIST1-mediated HCC development relies on PTEN expression, at least partially. The gene discussed is BHLHA15; the disease is hepatocellular carcinoma.