Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blotting (WB) assays showed that ARHGAP24 was downregulated in HCC cell lines with strong metastatic ability (HCCLM3 and MHCC97H), but was highly expressed in cells with weak metastatic potential (MHCC97L and Li-7) (Figure 2A). Here, ARHGAP24 is linked to hepatocellular carcinoma.