Co-immunoprecipitation combined with mass spectrometry showed that ARHGAP24 could serve as a scaffolding protein to promote the binding of the E3 ubiquitin ligase WWP1 to pyruvate kinase M2 (PKM2), and then degrade PKM2 through the ubiquitin-proteasome pathway to inhibit liver cancer invasion and metastasis. Here, ARHGAP24 is linked to liver cancer.