GUCY1A2 and esophageal squamous cell carcinoma: Similarly, Lasso regression analysis also revealed that a panel composed of three CNA loss-affected metabolic genes (SLN, ACAT1, and GUCY1A2) is associated with poor prognosis in patients with ESCC (0.0001, Kaplan-Meier survival analysis and Log-rank test, Figure 7F), also confirmed to be an independent prognostic factor by multivariate Cox regression survival analysis (p = 0.00136, HR = 1.92, 1.29 to 2.85, adjusted for age, gender, LNM, and drinking and smoking status, Figure 7G).