Most importantly, using molecular subtyping by CNA clusters, mutation panel (ADCY2, CACNA1D, GRIK2, KCNMA1, and KCNQ3), and genes with CNA loss panel (SLN, ACAT1, GUCY1A2), we stratified ESCC patients into distinct prognostic subgroups. The gene discussed is KCNMA1; the disease is esophageal squamous cell carcinoma.