Most importantly, using molecular subtyping by CNA clusters, mutation panel (ADCY2, CACNA1D, GRIK2, KCNMA1, and KCNQ3), and genes with CNA loss panel (SLN, ACAT1, GUCY1A2), we stratified ESCC patients into distinct prognostic subgroups. Here, GUCY1A2 is linked to esophageal squamous cell carcinoma.