Consistent with the mechanistic insights in previous reports53,54, flow cytometric analysis on the DC populations in the tumors of the BSA-Man@Mn2+-Ft@Lap group showed that their MHC-I and MHC-II expression levels have increased by 36.18% and 31.68% compared to the control group, supporting the enhanced capacity of these DCs to activate tumor-specific CD8 and CD4 T cells (Supplementary Fig. 22). The gene discussed is CD4; the disease is neoplasm.