Remarkably, western blot and immunofluorescence analysis revealed that CRT and HSP70 expression on 4T1 tumor cells increased by 1.6-fold and 1.5-fold after the incubation with BSA-Man@Mn2+-Ft@Lap compared to the control group (Fig. 3e–j and Supplementary Fig. 8), while ELISA assay of the supernatant consistently showed a 6- and 2.6-fold increase in HMGB1 and ATP release. Here, HMGB1 is linked to neoplasm.