Previous studies highlighted the role of epithelial-mesenchymal transition (EMT), characterized by down-regulated expression of E-cadherin and up-regulated expression of Vimentin, matrix metalloproteinases (MMPs), and Slug, in tumor migration and invasion.23–25 Our results showed that IFN-γ promotes the EMT of SACC-83, characterized by downregulation of E-cadherin, and upregulation of Vimentin, Slug and MMP9 (Fig. 2d). This evidence concerns the gene MMP9 and neoplasm.