It is worth noting that there was a significant discrepancy between Cluster A and Cluster B in terms of the distribution of FAB category, in which the AML-M0 subtype was only distributed in Cluster A, but not in Cluster B. Besides, more patients with NPM1 mutation distributed in Cluster B than Cluster A. However, there were no obvious differences between the patients in Cluster A and Cluster B with features of FLT3 mutation, IDH1 mutation, activating Ras, gender, age, fustat and futime. This evidence concerns the gene NPM1 and acute myeloid leukemia.