We found that RNase A treatment showed little effect on miR-452-5p level, but the additional treatment of Triton X-100 reduced miR-452-5p level significantly (Figure 3(b)), suggesting that miR-452-5p were encapsulated and protected against RNase A. Therefore, it was hypothesized that miR-452-5p selectively resided in the exosome lumen derived by HCC cells. The gene discussed is RNASE1; the disease is hepatocellular carcinoma.