TSA was used to estimate the sample size of meta‐analysis, and the result showed that the accumulation curve Z crossed the traditional boundary value, but did not cross the TSA boundary value, and its accumulated information did not reach the expected information (RIS, 8276), indicating that the meta‐analysis may have reached a false positive conclusion, and more studies need to be included to confirm the relationship between the expression of AFAP1‐AS1 and the prognosis of GC (Figure S2). Here, AFAP1 is linked to gastric cancer.