To prove that the CD44v6 expression in AML may be associated with FLT3 and other genetic mutations, we constructed SKM‐1‐FLT3 cells with FLT3/ITD mutation and SKM‐1‐DNMT3A‐SC2, SKM‐1‐DNMT3A‐SC3 cells with DNMT3A R882H mutation using SKM‐1 cells, respectively. This evidence concerns the gene FLT3 and acute myeloid leukemia.