MYC and colonic neoplasm: Out of the two E-boxes within the MDN1 promoter, mutation of the E-box closer to the transcription start site (TSS) (TSS +32) had an effect on cell proliferation (Fig. 2b), whereas the mutation of the E-box farther away (TSS −151) had no effect (Extended Data Fig. 5), despite MYC binding detected at both of these sites in HAP1 cells as well as using ChIP-nexus in colon cancer cells34 (Extended Data Fig. 4).