To test whether DHX30 interacts with TAR DNA-binding protein 43 (TDP-43), another ALS causative and RNA-binding protein, we performed an immunoprecipitation assay using FLAG antibody in HEK293A overexpressing TDP-43-FLAG WT, a familial ALS-linked mutant variant (Q331K), a cytoplasmic mislocalized artificial mutant (mNLS: R82L/K83Q), or an intracytosolic aggregate-forming mutant (mNLS/mRRM: C173S/C175S)34. Here, TARDBP is linked to amyotrophic lateral sclerosis.