RUNX2 and acute myeloid leukemia: To further evaluate whether in vitro GDYO treatment could affect in vivo leukemogenesis, we transplanted GDYO-treated OCI-AML3 cells into sublethally irradiated NOD/SCID/IL-2Rγ-null (NSG) mice, and found that pre-treatment of GDYO markedly reduced bioluminescence signals of the luciferase-expressing OCI-AML cells and prolonged the survival of engrafted mice (Supplementary Fig. 10).