To verify the impact of the HDAC7-c-Myc signaling on mediating ESCC proliferation and melatonin’s anti-proliferative activity, we examined the actions of knocking down c-Myc via further infecting shc-Myc lentivirus on the EC109 cells stably overexpressing HDAC7. This evidence concerns the gene MYC and esophageal squamous cell carcinoma.