SPP1 and glioblastoma: We exposed GBO samples from 1 patient, whose GBM tissue expressing high TMSB10, CD68 and SPP1, to CM from TMSB10 knockdown GSCs at the indicated time, and then treated the GSCs in combination with PD1 inhibitor (5 μM) and/or selumetinib (50 mg/ml) for 5 days.