To further verify the effect of TMSB10 knockdown on the immunosuppressive polarization of macrophages in vivo and to determine whether the combination of a TMSB10-targeted approach with selumetinib can improve the therapeutic effect on GBM, GSCs with TMSB10 knockdown or NC vectors were coimplanted with THP-1 cells in situ in the brains of nude mice, followed by treatment with or without selumetinib. This evidence concerns the gene TMSB10 and glioblastoma.