Moreover, we then performed an immunofluorescence staining assay with our local clinical glioma patient tissues, and the results showed that the expression of CD68 (a human macrophage marker) and SPP1 (a key inflammatory protein produced by TAMs that express the pattern recognition receptor MARCO and part of the largest group of proteins with cell type-specific expression) [29], was enhanced in TMSB10-high glioma tissues compared to TMSB10-low tissues (Fig. 7H). Here, SPP1 is linked to central nervous system cancer.