RPL34 and esophageal squamous cell carcinoma: To further investigate the expression pattern of RPL34-AS1 in ESCC, we also performed an analysis of RPL34-AS1 expression in a public microarray profile dataset from the Cancer Genome Atlas (TCGA) and used median RPL34-AS1 value as a cutoff, the results showed that RPL34-AS1 expression in 162 tumor samples of EC was significantly decreased, comparing with 11 adjacent tumor samples (Fig. 1D).