In current study, we discovered a potential target factor for preventing the occurrence and development of FLHS in laying hens; and further investigation certified that the inhibition of AMPK signal obviously exacerbated the excessive lipid accumulation, oxidative stress and inflammatory response triggered by PO in primary chicken hepatocytes, but activated AMPK signal presented obviously beneficial effects on PO-induced the excessive lipid accumulation, oxidative stress and inflammatory response in hepatocytes. Here, PRKAA1 is linked to Floating-Harbor syndrome.