PRKAA1 and obesity due to melanocortin 4 receptor deficiency: The results illustrated that the phosphorylation protein level of AMPKα (Thr172) was down-regulated in PO-stimulated primary chicken hepatocytes with a time-dependent manner (Figures 1A,B), which suggested that the inhibition of AMPK signal may be associated with the development of obesity-induced pathologies and that p-AMPKα (Thr172) may act a crucial role in this process.