To determine the role of AMPK signal on lipid metabolism disorders triggered by PO, the primary chicken hepatocytes were pre-treated with AMPK inhibitor compound C. Nile Red staining analysis showed that the excessive accumulation of lipid droplets induced by PO was markedly exacerbated in hepatocytes pre-treated with compound C (p < 0.01) (Figures 2A,B). The gene discussed is PRKAA1; the disease is Disorder of lipid metabolism.