The results illustrated that the phosphorylation protein level of AMPKα (Thr172) was down-regulated in PO-stimulated primary chicken hepatocytes with a time-dependent manner (Figures 1A,B), which suggested that the inhibition of AMPK signal may be associated with the development of obesity-induced pathologies and that p-AMPKα (Thr172) may act a crucial role in this process. The gene discussed is PRKAA1; the disease is obesity disorder.