To further confirm the reliability of the data of differentially expressed genes, the expression of 6 screened mRNAs (ILK, VCL, RRAS, TLN1, ITGA5, and PDGFRB) and 3 lncRNAs (n335645, n335724, and n339477) were detected by qRT-PCR in other 3 independent LCPD and 3 control tissues. The gene discussed is VCL; the disease is Legg-Calve-Perthes disease.