CP and Parkinson disease: Similarly, the mRFP-GFP-LC3 transfection method showed that compared with CP alone, PD significantly induced the increase of red puncta, but PD combined with CQ significantly increased the yellow puncta (with both GFP and RFP signals), suggesting that PD enhanced not only autophagosome formation but autophagy flux (Figures 3(f) and 3(g)).