This study showed that the autophagy flux was blocked in CP-treated HK-2 cells and CP-AKI mice, which was characterized by the increase of LC3 II and p62 in WB, and the decrease of autophagolysosome/autophagosome ratio in mRFP-GFP confocal test, which were consistent with other studies [37, 38]. The gene discussed is SQSTM1; the disease is acute kidney injury.