The results revealed that the protein and mRNA expressions of p-PI3K, p-P70S6k, p-mTOR, p-AKT, and HIF-1α in the miR-372-3p KD group were higher than those in the model and control groups, suggesting that miR-372-3p is an upstream target of the PI3K/AKT/mTOR/HIF-1α signaling pathway regulating angiogenesis in DCM mice. This evidence concerns the gene MTOR and familial dilated cardiomyopathy.