Among them, compound 25 showed better tumor cytotoxicity as a payload (A2780 IC50 = 5 nM), which demonstrated greater inhibitory activity against the following cells: NCI-H526 (high expression of c-kit antigen, IC50 = 2 nM), MDA-MB453 (high expression of HER2 antigen, IC50 = 0.4 nM) and NCI-N87 (high expression of HER2 antigen, IC50 = 1 nM). This evidence concerns the gene ERBB2 and neoplasm.