In IPITT assay, the effect of insulin on glucose clearance in diabetic mice was significantly lower than that in control mice, while metformin or JTSHF responded to the hypoglycemic efficiency of insulin after intraperitoneal injection of insulin, and had a remarkable clearance of their endogenous glucose compared with the mice in the DM group (Figures 3C,D, p < 0.05). The gene discussed is INS; the disease is diabetes mellitus.