AKT1 and cancer: Interestingly, PI3K, p-PI3K (Tyr 458), AKT1/2/3, and p-AKT1/2/3 were highly expressed in lung tumor metastases compared with healthy lung tissues, but their expression was significantly decreased in lung tumor metastases after Fx treatment (Figure 6C and Supplementary Figure S5), suggesting that Fx also inhibited PI3K/AKT signaling pathway in cancer cells in vivo.